People and animals Essay Example for Free

People and animals Essay Your role is to find a minimum of three connections between the reading and the world at large. This means connecting the reading to your own life, to happenings in the local and/or global community, to similar events at other times and places, to other people or problems which you are reminded. You might also see connections between this portion of the novel to other parts of it; other writings on the same topic, or similar issues being raised in other classes. Realize that whatever the reading connects you with is worth sharing. PLEASE TYPE YOUR RESPONSES. There are many connections to be made between Crichton’s Jurassic Park and life today. Michael Crichton is not exactly a scientific writer, but he uses real science to back up everything about his novels. He extrapolates the concepts, much as other science fiction writers do. The first example are the sentences â€Å"They are not free at all. They are essentially our prisoners. Unable to survive in real world† (Crichton). This reminds me of animals at a zoo or even the idea of cloning. Natural reproduction in animals is one thing, but making animals for only our purposes is questionable. I realize people breed dogs and other animals to sell, but large scale cloning becomes a little scary. In fact, there are some people now who believe in more rights for animals. These people would be very unhappy with a theme park that clones animals. They are already extremely unhappy with people who keep animals for pets. The next part that reminds me of something else is all the reliance on fences. The people who built Jurassic Park believe the fences will protect them. In the poem â€Å"Mending Wall,† Robert Frost says that before he built a fence, he would want to know what he is walling in or out. The same is true for this novel. First of all, the fence gives the characters a false sense of security. They believe the fence will protect them from the dinosaurs when, in reality, the dinosaurs can escape. However, in walling the dinosaurs out so heavily, they are also walling themselves in. It will be much harder for them to get away if anything would happen. I think we rely on all kinds of fences to give us a false sense of security. I think about gated communities. People buy expensive homes in gated communities to keep â€Å"undesirables† out, but really, it is not hard to leap a fence. If people want your wealth badly enough, they will go over the fence. The Mexican American border is another example. We believe that if we can staff the fence enough with la migra, we can protect ourselves from illegal immigration. The fact is that there is not enough fence to keep the United States from gaining thousands of illegal immigrants each year. Mexico is poverty, and the United States is wealth. Those fences won’t protect us at all. The whole concept of being able to visit a dinosaur park makes me think of eco-tourism and how popular it has become. We all want to get glimpses of true nature. We are willing to make our own land into parking lots, but we will travel to exotic places to see nature unspoiled. Unspoiled nature is not meant for tourists. Before long, garbage and refuse will be left in the rainforests or wherever and destroy the delicate balance of life there. Dinosaurs went extinct for a reason. Maybe we don’t know what the reason is, but they should not be brought back for our amusement. We should not travel to other countries to see animal life and primitive people. It is disrespectful. The last sentence to discuss is â€Å"The history of evolution is that life escapes all barriers† (Crichton). This is what we always forget in creating new things. Once we give something â€Å"life,† anything can happen. People and animals are not meant to be controlled and will always surprise us. The fact that the dinosaurs are breeding should not surprise anyone, but it does. The scientists actually believe that they can create an artificial environment that they can control. But adaptation is always in the works.

Business Ethics Essay (grapes -- essays research papers

Business Ethics Essay   Ã‚  Ã‚  Ã‚  Ã‚  Business ethics are a moral code that people conducting any sort of business should feel honorably obligated to follow. People are definitely morally responsible to follow a code of ethics and should never cheat each other. Parts of this code of ethics should be honesty, integrity, fidelity, charity, responsibility, and self-discipline. Those ethics should apply both to a person buying something, and a person selling something. I don’t believe that a person should be forced to follow the caveat emptor policy, on the basis that it is unfair and unjust both to the buyer and seller. If you are an ethical person, you should not have to warn people to beware.   Ã‚  Ã‚  Ã‚  Ã‚  A person selling something is morally obligated t...

Fixation and Fixatives

ALCIAN BLUE RATIONALE FOR USE Alcian blue is a water soluble, amphoteric copper phthalocyanine, which is most often used as a basic dye. In general, alcian blue has an affinity for sulfated and carboxylated groups of acid mucopolysaccharides, but the specific group demonstrated is dependent on the dye solution pH. RECOMMENDED FIXATIVES 10% neutral buffered formalin and Bouin’s fixative. AVOID Chromate Fixatives MODE OF ACTION (1, 2, 3) Alcian blue binds electrostatically with the acidic groups in the mucopolysaccharide molecule.The components to be demonstrated are intensely stained if the dyer solution is used at the specific pH at which the reactive groups are fully ionized. To achieve full ionization of the reactive groups, some alcian blue methods begin with a rinsing of sections in the appropriate pH solution prior to staining with the dye solution. In general, strongly sulfated mucins react more consistently at low pH levels. Above a pH of 1. 0 their reactions are variab le. At a pH of 0. 2 only strongly sulfated mucosubstances will be demonstrated, while weak sulfated mucins stain well between a pH of 1. 0 – 2. 5, and often below 1. . Carboxylated mucins react with alcian blue at pH 2. 5. Alcian blue produces an intense greenish-blue or teal coloration. To ensure this colour is not lost during subsequent chemical and dye treatments, such as those found in the Alcian Blue-Verhoeff’s van Gieson procedure and Movat’s Pentachrome, alcian blue is converted to an insoluble pigment. By exposing alcian blue stained sections to a pre-heated 80Â °C alkaline alcohol solution, alcian blue is converted to the insoluble pigment, monastral fast blue. Further exposure of the sections to various decolorizers and dyes, fails to change the alcian blue intensity.QUALITY CONTROL AND CONTROL MATERIALS 1. Appropriate control tissues include colon and small intestine. If lung sections contain bronchi with mucous glands, are used as a control, the tec h should be aware that cartilage matrix will also stain at approximately pH 2. 5. 2. To avoid coloration of hyaluronic acid found in connective tissue matrix or amorphous ground substance, the tissue section can first be treated with a solution of testicular streptococcal hyaluronidase before staining with alcian blue. Testicular hyaluronidase requires only 2 hours of incubation versus 24 hours with streptococcal hyaluronidase. . Alcian blue powder solubility may decrease after storage of more than three years, but good staining results have been reported with dye lots older than three years (4). 4. The dye solution pH is critical to ensure demonstration of specific chemical groups. pH 0. 2 -only strongly sulfated mucins demonstrated pH1. 0 -strongly and weakly sulfated mucins pH 2. 5 -carboxylated and weakly sulfated mucins 5. To ensure the quality of staining at the appropriate pH, the section may be rinsed in the solvent solution prior to staining in the dye, i. e. , rinsing in p H 2. , 3% aqueous acetic acid solution prior to placing the sections in the pH 2. 5 alcian blue solution, which is prepared by combining alcian blue dye powder in a 3% aqueous acetic acid solution. 6. Some procedures indicate it is important to avoid rinsing the stained slides in water after treatment in alcian blue dye. Blotting the slides dry after staining is recommended. 7. It is important to avoid celloidinization of tissue sections because alcian blue has a strong affinity for celloidin. 8. A white haze may appear on the back of the glass slide after staining with nuclear fast red and contact with the air.Moistening a kimwipe or facial tissue with saliva and rubbing the back of the glass will remove the haze. Follow this with cleaning of the glass using a kimwipe moistened with 100% ethanol to remove the saliva. 9. Cut paraffin sections at 4-6 microns. REFERENCES 1. Bancroft J. D. and Stevens A. : Theory and Practice of Histological Techniques, 2nd edition. Churchhill Livingst one, 1982. 2. Kiernan J. A. : Histological and Histochemical Methods: Theory and Practice, Pergamon Press, 1981. 3. Sheehan D. C. and Hrapchak B. B. : Theory and Practice of Histotechnology, 2nd edition, C.V. Mosby Co. , 1980. 4. Shrenk E. : Note from the Biological Stain Commission-a newly certified dye-Alcian blue 8GX. Stain. Tech. 56(3), 129, 1981. ALCIAN BLUE pH 2. 5 SOLUTIONS a. 1% Alcian Blue Solution – pH 2. 5 Alcian Blue 8GX (C. I. 74240)1gm 3% Glacial acetic acid100ml (3 ml. glacial acetic acid to 97 ml. distilled water) Add a crystal of thymol to prevent mold growth. The solution can be filtered and reused. b. Nuclear Fast Red (Kernechtrot) Solution (pg. 19) PROCEDURES (1, 2) 1. Deparaffinize and hydrate slides to distilled water. (See Note 1) 2.Stain in alcian blue solution for 30 minutes. Filter solution back for reuse. (See Note 2) 3. Wash for 2 minutes in running tap water. 4. Rinse in distilled water. 5. Counterstain in nuclear fast red for 3 to 5 minutes. Quic kly transfer slides to distilled water. Filter solution back for reuse. 6. Rinse slides in distilled water. 7. Dehydrate, clear, and mount. If a cloudy haze appears on the side refer to quality control information, #8. COLOR RESULTS Carboxylated and weakly sulfated acid mucins – light blue to medium greenish blue Nuclei-reddish pink.NOTE 1. After step 1, the slides may be placed in a pH 2. 5, 3% aqueous acetic solution for 1 minute, then proceed to step 2. 2. After step 2, the slides can be blotted dry, instead of rinsing in running tap water and distilled water. Continue the procedure with step 5. REFERENCES 1. Lev R. and Spicer S. S. : Specific staining of sulphate groups, with Alcian blue at low pH. J. Histochem. Cytochem. , 12:309, 1964. 2. Sheenhan D. C. and Hrapchak B. B. : Theory and Practice of Histotechnology, 2nd edition. C. V. Mosby Co. , 1980

Biology Case Study Essay - 624 Words

Case Study 1 1. Johnny has seen the words Sodium Chloride before in his science class with the periodic table. 2. Johnny couldn’t find Sodium Chloride on the periodic table because Sodium Chloride is a molecule made up of two atoms: Sodium and Chloride. 3. Sodium Chloride represents two atoms that together form a molecule: NaCl. Na: Sodium and Cl: Chloride 4. An ionic bond occurs when two or more ions (an atom whose number of elections differs from its number of protons) bond together because of their opposite charge. They are drawn to each other like magnets because of their opposite charge. 5. Sodium Chloride came about because Sodium, a positively charged ion, has one electron in its outermost shell and†¦show more content†¦This is important to remember with a water molecule because the hydrogen bonds between individual molecules and are constantly breaking and then reforming. This allows the molecules to pack close together. 11. Acids are any substance that yields hydrogen ions when put in aqueous solution. In other words, the concentration of hydrogen ions goes up the the further doe the pH scale you go. Bases are any substance that accepts hydrogen ions in aqueous solution. Again, the more basic it is the lower the concentration of hydrogen ions. 12. The pH scale is a scale used to measure t he relative acidity or alkalinity of a solution. The scale ranges from 0 to 14, and figures out the concentration of hydrogen ions in a solution. The lower the pH number, the more acidic the solution is. The higher the pH number, the more basic the solution. 13. At the chemical level, Johnny would be able to tell if something was an acid or a base by the concentration of hydrogen ions in a solution. 14. H+ refers to a hydrogen ion: a lone proton that has lost its electron making it a positively charged ion. 15. 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